Read the Paper: Comparing Culture Characteristics Between Acoustic Resonance Mixing and Orbital Shaking
Production of recombinant proteins usually happens in suspension cultures, with oxygen limitation playing a major role. We compared growth characteristics of microbial cultures in a standard shaken incubator with those of cultures in a RAMbio fermentation system, paying particular attention to oxygen transfer. The oxygen transfer rates of conventional orbital shakers are insufficient for optimizing microbial fermentations. By improving oxygen transfer as much as sixfold and cell densities as much as threefold, RAMbio systems enable dense cultures in less time than orbital shakers.
The RAMbio maximizes oxygenation in any flask – get more biomass, more protein, more plasmid,
- Eliminate oxygen limitations
- Uses traditional shake flasks
- Bioreactor-like performance from a simple flask
The RAMbio benchtop incubator uses Resonant Acoustic technology to get maximum performance from microbial cultures, thus replacing orbital shakers.
The RAMbio dramatically enhances oxygenation in bacterial, yeast, and fungal cultures. Using almost any shake flask, you can achieve 6-fold greater gas exchange, meaning you can achieve more than 300% more biomass and significant gains in product production.
Orbital Shakers Suffocate Cultures
Traditional shakers are terrible at aerating microbial cultures. Even with baffled flasks, orbital shakers only allow passive gas exchange. This means that the oxygen supply quickly becomes limited because the oxygen can't enter flasks as fast as the microbes need it.
The RAMbio Lets Your Cultures Breathe
When you use the Oxy-Pump stopper to close your flasks, the RAMbio actually pumps gas in and out of the headspace. Translation: Microbial cultures get all the oxygen they need and reach higher densities than available in orbital shakers.
Whether you're expressing protein, making large quantities of DNA, or just trying to increase biomass yields, the RAMbio is great for culturing strains with high oxygen demands:
If you rely on shake flasks to grow microbes, then you need to explore the RAMbio.
The RAMbio Incubator
The Oxy-Pump Stopper